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Anti-crotonyllysine mouse mAb (clone 4D5)

  • 服務簡介

    Product Description

    The pan anti-crotonyllysine monoclonal antibody (clone 4D5) is developed by using crotonylated-KLH conjugates as immunogen. The product specifically recognizes proteins with lysine crotonylated residues but not the structurally-similar modified lysine residues or non-modified lysine residues. Besides common applications in Western blotting, immunoprecipitation, immunofluorescence and immunohistochemistry, the antibody is with ChIP grade quality and has been well utilized for proteomic screening to lysine crotonylated substrates from protease-digested proteins both in prokaryotic and eukaryotic cells.??


    Source/Purification

    The pan anti-crotonyllysine monoclonal antibody (clone 4D5) is developed by using crotonylated-KLH conjugates as immunogen.


    Specificity

    All crotonylated proteins expected

    Recommended Applications

    ELISA, WB, IAP. Not tested in other applications

    *WB=Western blotting; IAP: Immuno affinity precipitation


    Recommended antibody dilution

    WB: 1:1000; IAP: 1:200

    Important

    For Western blotting, incubate membrane with diluted antibody in 5% BSA, 1 x TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight.

    Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications.

    Anti-crotonyllysine mouse mAb (clone 4D5)

    A.?Dot blotting?analysis on 10 ng of lysine crotonylated peptide library (A1), lysine crotonylated BSA (B1), selected histone lysine crotonylated peptides (A2-7, B2-6, C1-6), unmodified lysine peptide library (B7), unmodified BSA (C7), lysine acetylated peptide library (A8), lysine propionylated peptide library (B8), lysine butyrylated peptide library (C8) and randomly selected histone unmodified peptides (A9, B9, C9) using anti-crotonyllysine mAb (clone 4D5, 1:1000).


    B.?Western blotting?analysis?on 30?μg?of HeLa crude proteins using anti-crotonyllysine mAb (clone 4D5, 1:1000).


    Scientific Description

    Lysine crotonylation is a newly-identified histone post-translational modification by integrated proteomic approaches and elaborate biochemistry analyses. It has been shown that lysine crotonylation is an evolutionarily conserved PTM in multiple species of both prokaryotes and eukaryotes in a wide range of proteins, both in histones and non-histone substrates. The unique structure and genomic localization of lysine crotonylation suggest that it is mechanistically and functionally different from lysine acetylation. Given the revealed roles of lysine crotonylation in spermatogenesis, the novel modification may play important roles in multiple cellular pathophysiological processes by effecting chromatin structure and epigenetic pathways.


    Storage & Stability

    Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


    **Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



  • 技術原理

    Cat#:

    PTM-502

    Ab Type:

    M

    Applications:

    WB

    Species:

    All

    Price: (100 μl)

    3360.00

    Price: (20 μl)

    1008.00


  • 儀器設備

  • 技術優勢

    ?1.?Tan,?M.J.?et?al?(2011)?Cell?146?,1016

  • 應用領域

    ELISA, WB, IAP. Not tested in other applications

    *WB=Western blotting; IAP: Immuno affinity precipitation


  • 參考文獻

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