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組蛋白H3抗體
杭州景杰生物科技有限公司
Histone H3 rabbit pAb

Cat#:

PTM-1002

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

1380.00

Price: (20 μl)

414.00


Product Description

This product is produced by immunizing rabbits with a synthetic peptide corresponding to C terminus of human histone H3. Antibodies are purified by peptide affinity chromatography.


Source/Purification

This product is produced by immunizing rabbits with a synthetic peptide corresponding to C terminus of human histone H3. Antibodies are purified by peptide affinity chromatography.


Specificity

H3

Recommended Applications

ELISA, WB. Not tested in other applications?

*WB=Western blotting


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate ?membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for ?two hours at room temperature with gentle shaking.??

Use at an assay dependent concentration. Optimal ?dilutions/concentrations should be determined by the end user.??

Histone H3 rabbit pAb

? ?A.?Western blotting analysis on 30 μg of crude proteins from HeLa, C2C12, COS-7 whole cell lysates, and 200 ng recombinant histone H3 protein using histone H3 rabbit pAb (1:2000).


? ?B.?Western blotting analysis on 30 μg of crude proteins from mouse liver, mouse heart whole cell lysates using histone H3 rabbit pAb (1:500).


? ?C.?Western blotting analysis on 30?μg?of crude proteins from HeLa whole cell lysates with (right) or without (left) sodium butyrate treatment (30 mM, 4 hours) using histone H3 rabbit pAb (1:2000).The membrane was re-probed by propionyl-histone H3 (Lys14) rabbit pAb.


Scientific Description

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. It is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.


Storage & Stability

Store product at -20℃. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



ELISA, WB. Not tested in other applications?

*WB=Western blotting



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