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組蛋白賴氨酸三甲基化 (tri-methylation) 修飾抗體
杭州景杰生物科技有限公司
Anti-tri-methyl-Histone H3 (Lys27) mouse mAb

Cat#:

PTM-651

Ab Type:

M

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by tri-methylated histone H3 (Lys27) peptide affinity chromatography. It specifically recognizes histone H3 with tri-methylation at Lys27.??



Source/Purification

This product is produced by immunizing mice with a synthetic tri-methyl peptide corresponding to residues surrounding Lys27 of human histone H3. Antibodies are purified by protein G-conjugated agarose followed by tri-methylated histone H3 (Lys27) peptide affinity chromatography.??


Specificity

H3

Recommended Applications

ELISA, WB, IP; Not tested in other applications

*WB=Western blotting; IP= Immunoprecipation

?

Recommended antibody dilution

WB: 1:2000 IP: 1:25

Important

For Western blotting, incubate ?membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for ?two hours at room temperature with gentle shaking.??

Use at an assay dependent concentration. Optimal ?dilutions/concentrations should be determined by the end user.??

?Anti-tri-methyl-Histone H3 (Lys27) mouse mAb?

?

A.Dot ?blotting analysis on indicated amount of tri-methylated H3 peptide at Lys27 ?(lane 1), mono-methylated H3 peptide at Lys23(lane 2), mono-methylatedH3 ?peptide at Lys27 (lane 3), di-methylatedH3 peptide at Lys27 (lane 4) and unmodified H3 peptide at Lys27 (lane 5)using tri-methyl-histone H3 (Lys27)mouse ?mAb.


B.Western blotting?analysis on 30 μg of crude proteins from HeLa,C2C12 and COS whole cell ?lysates using tri-methyl-histone H3 (Lys27)mouse mAb(1:2000).??


Scientific Description

The ε-amino lysine methylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine methylation in multiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation in core histones is a major determinant for theformation of active and inactive regions of the genome and therefore playsvital roles in multiple cellular events. In most species, lysine methylationoccurs primarily on histones H3 (Lys4, 9, 27, 36, 79), and H4 (Lys20) and hasbeen implicated in both transcriptional activation and silencing. Methylation in histones modulated by specific histone methylases (HMTs) and histone demethylases (HDMs). The level of methyllysineis found impaired in the pathologies of cancers and other diseases, therefore, enzymes regulating histone lysine methylation have become promising targets for anti-cancer drugs.??


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.?

?

**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.?


ELISA, WB, IP; Not tested in other applications

*WB=Western blotting; IP= Immunoprecipation


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