科技產品 Products
組蛋白精氨酸甲基化 (arg-methylation) 修飾抗體
杭州景杰生物科技有限公司
Anti-di-methyl(sy)-Histone H4 (Arg3) rabbit pAb

Cat#:

PTM-639

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by symmetric di-methylated histone H4 (Arg3) peptide affinity chromatography. It specifically recognizes histone H4 with symmetric di-methylation at Arg3.

Source/Purification

This product is produced by immunizing rabbits with a synthetic symmetric di-methyl peptide corresponding to residues surrounding Arg3 of human histone H4. Antibodies are purified by protein A-conjugated agarose followed by symmetric di-methylated histone H4 (Arg3) peptide affinity chromatography.?

Specificity

H3

Recommended Applications

ELISA, WB, IP; Not tested in other applications??

*WB=Western blotting; IP= Immunoprecipation


Recommended antibody dilution

WB: 1:2000 IP: 1:50

Important

For Western blotting, ?incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% ?Tween-20 for two hours at room temperature with gentle shaking.??

Use at an assay dependent ?concentration. Optimal dilutions/concentrations should be determined by the end ?user.???

Anti-di-methyl(sy)-Histone H4 (Arg3) rabbit pAb

A.?Dot ?blotting analysis on indicated amount of symmetric di-methylated H4 peptide at Arg3 ?(lane 1), asymmetric di-methylated H4 peptide at Arg3 (lane2), acetylated H4 ?peptide at Lys5 (lane3) and non-methylated H4 peptide at Arg3 (lane 4) using symmetric ?di-methyl-histone H4 (Arg3) rabbit pAb.

?

B.?Western blotting analysis on 30 μg of crude proteins ?from HeLa, A431, C2C12, AML-12 and COS whole cell lysates using symmetric di-methyl-histone ?H4 (Arg3) rabbit pAb (1:2000).??



Scientific Description

Proteins post-translational Modification (PTM) is an important reversible event controlling protein activity. The amino-terminal tails of core histones undergo multiple modifications in multiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation or arginine methylation in core histones is a major determinant for the formation of active and inactive regions of the genome and therefore plays vital roles in multiple cellular events. In most species, arginine methylation occurs primarily on histones H3 (Arg2, 8, 17, 26) and H4 (Arg3) and has been implicated in both transcriptional activation and silencing. Arginine methylation in histones is carried out by the arginine N-methyltransferase (PRMT), which catalyzes the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a guanidine nitrogen of arginine. Arginine methylation in histones is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone arginine methylation have become promising targets for anti-cancer drugs.??

Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



??1. Mavrakis, K.J. et al, Science. 2016 Mar 11; 351(6278):1208-13.??

ELISA, WB, IP; Not tested in other applications??

*WB=Western blotting; IP= Immunoprecipation


推薦產品 PRODUCTS
Copyright ?2018-2020 杭州景杰生物科技有限公司
犀牛云提供云計算服務
X
合作
交流
1

QQ設置

3

SKYPE 設置

4

阿里旺旺設置

等待加載動態數據...

等待加載動態數據...

5

電話號碼管理

  • 400-100-1145
6

二維碼管理

等待加載動態數據...

等待加載動態數據...

展開
合作交流
填寫信息,我們的專屬學術顧問將為您解答疑問!
江苏快三计划网大小 河南省| 沙洋县| 佛冈县| 漾濞| 肥西县| 津市市| 玉山县| 金门县| 富阳市| 西吉县| 兴和县| 乃东县| 攀枝花市| 阿合奇县| 盐边县| 隆昌县| 麻城市| 东安县| 盐池县| 萝北县| 克什克腾旗| 广宗县| 宣城市| 荥经县| 泊头市| 砀山县| 高雄市| 宜州市| 招远市| 甘肃省| 东兴市| 明溪县| 疏附县| 罗源县| 沙坪坝区| 确山县| 华蓥市| 达日县|