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組蛋白精氨酸甲基化 (arg-methylation) 修飾抗體
杭州景杰生物科技有限公司
Anti-di-methyl(asy)-Histone H3(Arg2) mouse mAb

Cat#:

PTM-668

Ab Type:

M

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by symmetric di-methylated histone H2A at arginine3 peptide affinity chromatography. It specifically recognizes histone H2A with symmetric di-methylation at Arg3.??


Source/Purification

This product is produced by immunizing rabbitswith a synthetic di-methyl peptide?corresponding to residues surrounding Arg3 of human histone H2A. Antibodies are purified by protein A-conjugated agarose followed by symmetric di-methylated histone H2A at Arg3 peptide affinity chromatography.??


Specificity

H2A

Recommended Applications

ELISA, WB, IP; Not tested in other applications??

*WB=Western blotting; IP= Immunoprecipation


Recommended antibody dilution

WB: 1:2000 IP: 1:50

Important

?For Western blotting, ?incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% ?Tween-20 with gentle shaking two hours at room temperature.??

?Use at an assay dependent ?concentration. Optimal dilutions/concentrations should be determined by the end ?user.Not yet tested in other applications.??

?Anti-di-methyl(asy)-Histone H3(Arg2) mouse mAb

?A.?Dot blotting analysis on indicated amount of symmetric di-methylated H2A ?peptide at Arg3 (lane 1), asymmetric di-methylated H2A peptide at Arg3 (lane 2), ?acetylated H2A peptide at Lys4 (lane 3) and unmodified H2A peptide at Arg3 ?(lane 4) using di-methyl(sy)-histone H2A (Arg3)rabbit pAb.

?

B.Western blotting analysis on 30 μg of crude proteins from HeLa, C2C12, COS-7 whole?cell lysates and 200 ng recombinant histone H2A proteins using symmetric di-methyl-histone H2A (Arg3)rabbit pAb (1:2000).


Scientific Description

Proteins post-translational Modification (PTM) is an important reversible event controlling protein activity. The amino-terminal tails of core histones undergo multiple modifications inmultiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation or arginine methylation in core histones is a major determinant for the formation of active and inactive regions of the genome and therefore plays vital roles in multiple cellular events. In most species, arginine methylation occurs primarily on histones H3 (Arg2, 8, 17, 26) and H2A (Arg3) and has been implicated in both transcriptional activation and silencing. Arginine methylation in histones is carried out by the arginine N-methyltransferase (PRMT), which catalyzes the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a guanidine nitrogen of arginine. Arginine methylation in histones is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone arginine methylation have become promising targets for anti-cancer drugs.??


Storage & Stability

Store product at -20°C. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


ELISA, WB, IP; Not tested in other applications??

*WB=Western blotting; IP= Immunoprecipation


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