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組蛋白賴氨酸乙酰化 (acetylation) 修飾抗體
杭州景杰生物科技有限公司
Anti-acetyl-Histone H3 (Lys122) rabbit pAb

Cat#:

PTM-184

Ab?Type:

P

Application:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100ul)

3060

Price: (20ul)

700

Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by acetylated histone H3 (Lys122) peptide affinity chromatography. It specifically recognizes histone H3 with acetylation at Lys122.


Source/Purification

This product is produced by immunizing rabbits with a synthetic acetyl peptide corresponding to residues surrounding Lys122 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by acetylated histone H3 (Lys122) peptide affinity chromatography.


Specificity

H3

Recommended Applications

ELISA, Dot blot, Western blot.?

Recommended antibody dilution: WB: 1:2000


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate ?membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for ?two hours at room temperature with gentle shaking.??

Use at an assay dependent concentration. Optimal ?dilutions/concentrations should be determined by the end user.??

image.png

A. Dot blotting analysis?on indicated amount of peptides using histone H3K122ac rabbit pAb. The list of peptides is included in the table on the right.



1526299548233955.jpg

? ? ? ? ? ? ? ? ? ? ? ? ? ?

Western blotting analysis?on 30 μg of crude proteins from human HeLa whole cell lysates with (right) or without (left) treatment of sodium butyrate (30 mM, 4 hours)?(Fig. B)?or on 30 μg of crude proteins from JK and BRL whole cell lysates(Fig. C)??using acetyl-histone H3 (Lys122) rabbit pAb (1: 2000).?

?

Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code”. Lysine acetylation in core histones occurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation, and nuclear import. In most species, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of histone lysine acetylation status is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs.


Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.



ELISA, Dot blot, Western blot.?

Recommended antibody dilution: WB: 1:2000



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