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組蛋白賴氨酸甲基化(methylation) 修飾抗體
杭州景杰生物科技有限公司
Anti-tri-methyl-Histone H3 (Lys4) rabbit pAb ChIP Grade

Cat#:

GC-623

Ab Type:

P

Applications:

ChIP, IP

Species:

Eukaryotes

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by tri-methylated histone H3 (Lys4) peptide affinity chromatography. It specifically recognizes histone H3 with lysine tri-methylation at Lys4.


Source/Purification

This product is produced by immunizing rabbits with a synthetic tri-methyl peptide corresponding to residues surrounding Lys4 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by tri-methylated histone H3 (Lys4) peptide affinity chromatography.


Specificity

H3

Recommended Applications

ChIP, IP. Not tested in other applications?

*IP=Immunoprecipitation ? ? ? ?ChIP=Chromatin precipitation


Recommended antibody dilution

IP: 1:100 ChIP: 1:100 (1 μl of antibody for 1x 105 HeLa)

Important

?Use ?at an assay dependent concentration. Optimal dilutions/concentrations should be ?determined by the end user.??

?Anti-tri-methyl-Histone H3 (Lys4) rabbit pAb ChIP Grade??

A.?Dot ?blotting analysis on indicated amount of tri-methylated H3 peptide at Lys4 ?(lane 1), mono-methylated H3 peptide at Lys4 (lane 2), di-methylated H3 peptide ?at Lys4 (lane 3), mono-methylated H3 peptide at Lys9 (lane 4), di-methylated H3 ?peptide at Lys9 (lane 5), tri-methylated H3 peptide at Lys9 (lane 6), ?mono-methylated H3 peptide at Lys14 (lane 7), di-methylated H3 peptide at Lys14 ?(lane 8), tri-methylated H3 peptide at Lys14 (lane 9) and non- tri-methylated ?H3 peptide at Lys4 (lane 10) using tri-methyl-histone H3 (Lys4) rabbit pAb.?


B.?Sonicated chromatin prepared from 1 x 105?HeLa cells was ?subjected to chromatin immunoprecipitation (ChIP) using either serial dilutions ?of Tri-methyl-Histone H3 (Lys4) rabbit ?pAb or 1 μg of normal rabbit IgG?as a negative control. Real time quantitative PCR was ?performed on immunoprecipitated DNA using primers specific for the human?β-globin, MyoD1 Exon 1, RPL30 Exon 3, and GAPDH ?Promoter. Data are presented as enrichment of each IPed sample relative to ?total amount of input chromatin at each amplicon.


Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine methylation in multiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation in core histones is a major determinant for the formation of active and in active regions of the genome and therefore plays vital roles in multiple cellular events. In most species, lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing. Methylation in histones modulated by specific histone methylases (HMTs) and histone demethylases (HDMs) is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine methylation have become promising targets for anti-cancer drugs.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



ChIP, IP. Not tested in other applications?

*IP=Immunoprecipitation ? ? ? ?ChIP=Chromatin precipitation

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