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組蛋白賴氨酸甲基化(methylation) 修飾抗體
杭州景杰生物科技有限公司
Anti-di-methyl-Histone H3 (Lys9) mouse mAb ChIP Grade

Cat#:

GC-654

Ab Type:

M

Applications:

ChIP, IP

Species:

Eukaryotes

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by di-methylated histone H3 (Lys9) peptide affinity chromatography. It specifically recognizes histone H3 with lysine di-methylation at Lys9.


Source/Purification

This product is produced by immunizing mice with a synthetic di-methyl peptide corresponding to residues surrounding Lys9 of human histone H3. Antibodies are purified by protein G-conjugated agarose followed by di-methylated histone H3 (Lys9) peptide affinity chromatography.


Specificity

H3

Recommended Applications

ChIP, IP. Not tested in other applications?

*IP=Immunoprecipitation ? ? ? ?ChIP=Chromatin precipitation


Recommended antibody dilution

IP: 1:25, ChIP: 1:25 (4 μl of antibody for 1x 105 HeLa)

Important

?Use ?at an assay dependent concentration. Optimal dilutions/concentrations should be ?determined by the end user. Not yet tested in other applications.??

Anti-di-methyl-Histone H3 (Lys9) mouse mAb ChIP Grade

A.?Dot ?blotting analysis on indicated amount of di-methylated H3 peptide at Lys9 (lane ?1), mono-methylated H3 peptide at Lys9 (lane 2), tri-methylated H3 peptide at Lys9 ?(lane 3), mono-methylated H3 peptide at Lys4 (lane 4), di-methylated H3 peptide ?at Lys4 (lane5), tri-methylated H3 peptide at Lys4 (lane6), ?and non-methylated H3 peptide at Lys9 (lane 7) ?using di-methyl-histone H3 (Lys9) mouse mAb.??


B.?Sonicated ?chromatin prepared from 1x 105?HeLa cells was subjected to chromatin immunoprecipitation (ChIP) using ?either 4 μl di-methyl-histone H3 (Lys9) mouse ?mAb or 1 μg of normal mouse IgG as a negative control. Real time quantitative ?PCR was performed on immunoprecipitated DNA using primers specific for the ?human?GAPDH?promoter, GAPDH Coding D2?and?α Satellite. Data are presented as ?enrichment of each IPed sample relative to total amount of input chromatin at ?each amplicon.


Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine methylation in multiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation in core histones is a major determinant for the formation of active and inactive regions of the genome and therefore plays vital roles in multiple cellular events. In most species, lysine methylation which occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20), has been implicated in both transcriptional activation and silencing. Methylation in histones modulated by specific histone methylases (HMTs) and histone demethylases (HDMs) is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine methylation have become promising targets for anti-cancer drugs.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


ChIP, IP. Not tested in other applications?

*IP=Immunoprecipitation ? ? ? ?ChIP=Chromatin precipitation


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