科技產品 Products
賴氨酸乙酰化 (acetylation) 修飾ChIP級抗體
杭州景杰生物科技有限公司
Anti-acetyl-Histone H3 (Lys9) rabbit pAb ChIP Grade

Cat#:

GC-122

Ab Type:

P

Applications:

ChIP, IP

Species:

Eukaryotes

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by acetylated histone H3 (Lys9) peptide affinity chromatography. It specifically recognizes histone H3 with acetylation at Lys9.


Source/Purification

This product is produced by immunizing rabbits with a synthetic acetyl peptide corresponding to residues surrounding Lys9 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by acetylated histone H3 (Lys9) peptide affinity chromatography.


Specificity

H3

Recommended Applications

ChIP, IP. Not tested in other applications

*IP=Immunoprecipitation ? ? ? ?ChIP=Chromatin precipitation


Recommended antibody dilution

IP: 1:25 ChIP: 1:25 (4 μl of antibody for 1x 105 HeLa)

Important

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user.??

?Anti-acetyl-Histone H3 (Lys9) rabbit pAb ChIP Grade

A.?Dot blotting analysis on indicated amount of acetylated H3 peptide at Lys9 (lane 1), butyrylated H3 peptide at Lys9 (lane 2), crotonylated peptide at Lys9 (lane 3) and unmodified H3 peptide at Lys9 (lane 4) using acetyl-histone H3 (Lys9) rabbit pAb.


B.?Sonicated chromatin prepared from 1x 105 HeLa cells treated with sodium butyrate was subjected to chromatin immunoprecipitation (ChIP) using either serial dilutions of Acetyl-Histone H3 (Lys9) pAb or 1 μg of normal rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human β-globin, MyoD1 Exon 1, GAPDH Promoter and RPL30 Exon 3. Data are presented as enrichment of each IPed sample relative to total amount of input chromatin at each amplicon.


Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code”. Lysine acetylation in core histone s occurs in response to various stimuli and plays vital roles in the egulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation, and nuclear import. In most species, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15, 16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of histone lysine acetylation status is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.

?

**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.??


ChIP, IP. Not tested in other applications

*IP=Immunoprecipitation ? ? ? ?ChIP=Chromatin precipitation


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