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賴氨酸乙酰化 (acetylation) 修飾ChIP級抗體
杭州景杰生物科技有限公司
Anti-acetyl-Histone H2B (Lys20) mouse mAb ChIP Grade

Cat#:

GC-165

Ab Type:

M

Applications:

ChIP, IP

Species:

Eukaryotes

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description
?The mouse-derived antibody is purified with protein G-conjugated agarose followed by acetylated histone H2B (Lys20) peptide affinity chromatography. It specifically recognizes histone H2B with lysine acetylation at Lys20.
??
Source/Purification
?This product is produced by immunizing mice with a synthetic acetyl peptide corresponding to residues surrounding Lys20 of human histone H2B. Antibodies are purified by protein G-conjugated agarose followed by acetylated histone H2B (Lys20) peptide affinity chromatography.??
Specificity

H3

Recommended Applications

?ChIP, IP. Not tested in other applications???

?*IP=Immunoprecipitation ? ? ? ?ChIP=Chromatin precipitation??


Recommended antibody dilution

IP: 1:25 ChIP: 1:25 (4 μl of antibody for 1x 105 HeLa)

Important

?Use ?at an assay dependent concentration. Optimal dilutions/concentrations should be ?determined by the end user.??

?Anti-acetyl-Histone H2B (Lys20) mouse mAb ChIP Grade

?A. Dot ?blotting analysis?on indicated amount of acetylated H2B peptide at Lys20 (lane 1), ?acetylated H2B peptide at Lys16 (lane 2), crotonylated H2B peptide at Lys20 ?(lane 3), butyrylated H2B peptide at Lys20 (lane 4) and unmodified H2B peptide ?at Lys20 (lane 5) using acetyl-histone H2B (Lys20) mouse mAb.??


?B.Sonicated chromatin?prepared from 1x 105 HeLa cells treated with sodium butyrate was subjected to chromatin ?immunoprecipitation (ChIP) using either serial dilutions of Acetyl-Histone H2B (Lys20) mouse mAb or 1 μg of normal mouse IgG ?as a negative control. Real time quantitative PCR was performed on ?immunoprecipitated DNA using primers specific for the human β-globin, RPL30 Exon 3 and MyoD1 Exon 1. ?Data are presented as enrichment of each IPed sample relative to total amount ?of input chromatin at each amplicon. ?

?

Scientific Description
?The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code”. Lysine acetylation in core histones occurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation and nuclear import et al. In most species, histone H2A is primarily acetylated at Lys5, 9, 15 and 36; H2B is primarily acetylated at Lys5, 12, 15, 16 and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56 and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16 and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of histone lysine acetylation status is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs.??
Storage & Stability

?Store product at -20oC. Avoid repeated freeze/thaw cycle. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.??

?

?**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.??


?ChIP, IP. Not tested in other applications???

?*IP=Immunoprecipitation ? ? ? ?ChIP=Chromatin precipitation??


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