科技產品 Products
β-Tubulin抗體
杭州景杰生物科技有限公司
β-Tubulin mouse mAb

Cat#:

PTM-1011

Ab Type:

M

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

1380.00

Price: (20 μl)

414.00


Scientific Description:

Alpha and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes, including mitosis. Tubulin-binding drugs kill cancerous cells by inhibiting microtubule dynamics, which are required for DNA segregation and therefore cell division.

In eukaryotes there are six members of the tubulin superfamily, although not all are present in all species. Both α and β tubulins have a mass of around 50 kDa and are thus in a similar range compared to actin (with a mass of ~42 kDa). In contrast, tubulin polymers (microtubules) tend to be much bigger than actin filaments due to their cylindrical nature.?


β-Tubulin mouse mAb

Western blotting?analysis on 30 μg of crude proteins from HeLa, BRL, COS-7, N2a whole cell lysates and mouse liver, kidney whole tissue lysates?using?β-Tubulin mouse?mAb (1:2000).



Storage & Stability:?

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.


β-Tubulin mouse mAb


Source/Purification:??

This product is produced by immunizing Balb/c?mice with a synthetic peptide corresponding to N?terminus of β-Tubulin.?Antibodies are purified by protein G-conjugated agarose.


Recommended Applications:??

ELISA,?Dot blot, Western blot. Recommended antibody dilution: WB: 1:2000


NOTE:?For WB, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for?two hours at room temperature?with gentle shaking. Prepare working dilution immediately before use.?Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications


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