科技產品 Products
賴氨酸丁酰化 (butyrylation) 抗體
杭州景杰生物科技有限公司
Anti-butyryllysine mouse mAb

Cat#:PTM-329Ab Type:M

Applications:WBSpecies:All

Price: (100 μl):¥?3360.00? ? ? ? ? ? ? ??Price: (20 μl):¥?1008.00









Product Description
Source/Purification

This product is produced by immunizing mice with butyrylated BSA. Antibodies are purified by butyrylated-lysine agarose affinity chromatography.


Specificity

All

Recommended Applications

ELISA, WB

*WB=Western blotting


Recommended antibody dilution

WB: 1:1,000

Important

For Western blotting, incubate ?membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for ?two hours at room temperature with gentle shaking.??

Use at an assay dependent concentration. Optimal ?dilutions/concentrations should be determined by the end user.??


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Scientific Description

Butyrylation of lysine, structurally similar to lysine acetylation and lysine propionylation, is a newly identified reversible modification controlling protein activity. With integrated proteomic approaches and biochemistry analysis, lysine butyrylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates. Given the facts that many lysine residues in histones and non-histone substrates, such as p53, p300/CBP, are butyrylated, lysine butyrylation may play a vital role in epigenetic modulation by impacting on chromatin dynamics and plasticity, DNA transcriptional regulation and tumorigenesis, etc.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only.?Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



1. Zhang K, Chen Y, Zhang Z, et al. J Proteome Res. 2009, 8(2):900-906

2. Cheng Z, Tang Y, Chen Y, et al. Mol Cell Proteomics. 2009, 8(1):45-52.

3. Chen Y, Sprung R, Tang Y, et al. Mol Cell Proteomics. 2007, 6(5):812-819.4.


ELISA, WB

*WB=Western blotting


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