科技產品 Products
賴氨酸戊二酰化(glutarylation)修飾抗體
杭州景杰生物科技有限公司
Anti-glutaryllysine rabbit pAb

Cat#:

PTM-1151

Ab Type:

P

Applications:

WB

Species:

All

Price: (100 μl)

3680.00

Price: (20 μl)

1104.00


Product Description

The pan polyclonal anti-glutaryllysine antibody is produced by immunizing rabbits with glutarylated BSA. Antibodies are purified by glutaryl-lysine agarose affinity chromatography. The product specifically recognizes proteins with lysine glutarylated residues but not the structurally-similar modified lysine residues or non-modified lysine residues. Besides common applications in Western blotting, the antibody has been well utilized for proteomic screening to lysine glutarylated substrates from protease-digested proteins both in prokaryotic and eukaryotic cells.??


Source/Purification

Anti-glutaryllysine rabbit pAbpan polyclonal anti-glutaryllysine antibody is produced by immunizing rabbits with glutarylated BSA. Antibodies are purified by glutaryl-lysine agarose affinity chromatography.

?

Specificity

All

Recommended Applications

WB, IAP???

*WB=Western blotting; ?IAP=Immuno affinity preciptation??


Recommended antibody dilution

WB: 1:2000

Important

?For ?Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 ?x TBS, 0.1% Tween-20 at room temperatue with gentle shaking two hours.???

?Use at an assay dependent concentration. ?Optimal dilutions/concentrations should be determined by the end user.??

?

Anti-glutaryllysine rabbit pAb

?

?A.Dot blotting analysis?on glutarylated ?peptide library (Lane 1), succinylated peptide library (Lane 2), malonylated ?peptide library (Lane 3), acetylated peptide library (Lane 4) and unmodified ?peptide library using anti-glutaryllysine rabbit pAb.???

?

?B.Western blotting analysis?on 30 μg of crude proteins from human HeLa, mouse MEF, Drosophila S2, yeast S. cerevisiae and bacteria E. coli whole cell lysates using anti-glutaryllysine rabbit pAb (1: 1000).?


Scientific Description

Lysine glutarylation is a newly-identified histone post-translational modification by integrated proteomic approaches and elaborate biochemistry analyses. It has been shown that lysine glutarylation is an evolutionarily conserved PTM in multiple species of both prokaryotes and eukaryotes in a wide range of proteins, both in histones and non-histone substrates. Lysine glutarylation is a SIRT5- and nutrient-regulated PTM, which impacts metabolic processes and other mitochondrial functions.


Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.



1.Tan M,et al. Lysine glutarylation is a protein posttranslational modification regulated by SIRT5.Cell Metab. 2014 Apr 1;19(4):605-17. doi: 10.1016/j.cmet.2014.03.014.

2. Sadhukhan S,et al. Metabolomics-assisted proteomics identifies succinylation and SIRT5 as important regulators of cardiac function.Proc Natl Acad Sci U S A. 2016 Apr 19;113(16):4320-5. doi: 10.1073/pnas.1519858113. Epub 2016 Apr 5.

3. Zhou L,et al.SIRT5 promotes IDH2 desuccinylation and G6PD deglutarylation to enhance cellular antioxidant defense.EMBO Rep. 2016 Jun;17(6):811-22. doi: 10.15252/embr.201541643. Epub 2016 Apr 9.

4. Lin JB,et al.NAMPT-Mediated NAD(+) Biosynthesis Is Essential for Vision In Mice.Cell Rep. 2016 Sep 27;17(1):69-85. doi: 10.1016/j.celrep.2016.08.073.



WB, IAP???

*WB=Western blotting; ?IAP=Immuno affinity preciptation??


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