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杭州景杰生物科技有限公司
Anti-sumo1 mouse mAb

Cat#:

PTM-1110

Ab Type:

M

Applications:

WB

Species:

Human, Mouse, Rat, Monkey

Price: (100 μl)

2880.00

Price: (20 μl)

864.00


Product Description

This product is produced by immunizing Balb/c mice with a synthetic peptide from the carboxyl terminus of human SUMO1. Antibodies are purified by protein G-conjugated agarose followed by SUMO1 by peptide affinity chromatography.



Source/Purification

This product is produced by immunizing Balb/c mice with a synthetic peptide from the carboxyl terminus of human SUMO1. Antibodies are purified by protein G-conjugated agarose followed by SUMO1 by peptide affinity chromatography.


Specificity


Recommended Applications

ELISA, Dot blot, Western blot. Recommended antibody dilution: WB: 1:2000


Recommended antibody dilution

WB: 1:2000

Important

QQ截圖20171108095236.png

Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (right) or without (left) treatment of MG-132 (10 μM for 1 hour) (Figure A) and 30 μg of crude proteins from N2a, BRL, COS-7, MCF-7 whole cell lysates and mouse liver whole tissue lysates (Figure B) using SUMO1 mouse mAb (1: 2000).


Scientific Description

The functions of the small ubiquitin-related modifier 1 (sumo1) protein is similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. It can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development.?


Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.


ELISA, Dot blot, Western blot. Recommended antibody dilution: WB: 1:2000

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