科技產品 Products
賴氨酸丙酰化 (propionylation) 抗體
杭州景杰生物科技有限公司
Anti-propionyllysine antibody

Cat#:

PTM-201

Ab Type:

P

Applications:

WB

Species:

All

Price: (100 μl)

3360.00

Price: (20 μl)

1008.00


Product Description

The rabbit-derived pan propionyllysine antibody is purified with propionylated-lysine agarose affinity chromatography. It specifically recognizes proteins with propionyllysine residues but not the acetyllysine or butyryllysine residues with structural similarity. Except the common usage such as Western blotting, immunoprecipitation and immunofluorescence, the antibody is capable of capturing lysine propionylated peptides from protease-digesting proteins both in prokaryotic and eukaryotic cells in systematically proteomic screening.??


Source/Purification

This product is produced by immunizing rabbits with propionylated BSA. Antibodies are purified by propionylated-lysine agarose affinity chromatography.??


Specificity

All

Recommended Applications

ELISA, WB, IP?

*WB=Western blotting; IP=Immunopreciptation


Recommended antibody dilution

WB: 1:1,000; IP: 1: 25-1:100

Important

?For Western blotting, incubate ?membrane with diluted antibody in 5% BSA, 1 x TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight.??

? Use at an assay dependent ?concentration. Optimal dilutions/concentrations should be determined by the end ?user.?Not yet tested in other applications.??

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Scientific Description

Propionylation of lysine, structurally similar to lysine acetylation, is an newly identified reversible modification controlling protein activity. The reversible lysine propionylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates, such as p53. It is speculated that lysine propionylation plays a vital role in the regulation of multiple cellular processes including chromatin dynamics and plasticity and DNA transcriptional regulation by sharing same regulative enzymes with lysine acetylation or with its unique regulative enzymes.??


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



Zhang K,et al.Identification and verification of lysine propionylation and butyrylation in yeast core histones using PTMap software.J Proteome Res. 2009 Feb;8(2):900-6. doi: 10.1021/pr8005155.

Gattner MJ,et al.Synthesis of ε-N-propionyl-, ε-N-butyryl-, and ε-N-crotonyl-lysine containing histone H3 using the pyrrolysine system.Chem Commun (Camb). 2013 Jan 14;49(4):379-81. doi: 10.1039/c2cc37836a. Epub 2012 Nov 28.

Fritz KS,et al.Ethanol metabolism modifies hepatic protein acylation in mice.PLoS One. 2013 Sep 20;8(9):e75868. doi: 10.1371/journal.pone.0075868. eCollection 2013.

Pougovkina O,et al.Aberrant protein acylation is a common observation in inborn errors of acyl-CoA metabolism.J Inherit Metab Dis. 2014 Sep;37(5):709-14. doi: 10.1007/s10545-014-9684-9. Epub 2014 Feb 15.

Du Y,et al.Lysine malonylation is elevated in type 2 diabetic mouse models and enriched in metabolic associated proteins.Mol Cell Proteomics. 2015 Jan;14(1):227-36. doi: 10.1074/mcp.M114.041947. Epub 2014 Nov 23. Singhal A,et al.Systematic Analysis of Mycobacterial Acylation Reveals First Example of Acylation-mediated Regulation of Enzyme Activity of a Bacterial Phosphatase.J Biol Chem. 2015 Oct 23;290(43):26218-34. doi: 10.1074/jbc.M115.687269. Epub 2015 Sep 8.

Cheng Z,et al.Molecular characterization of propionyllysines in non-histone proteins.Mol Cell Proteomics. 2009 Jan;8(1):45-52. doi: 10.1074/mcp.M800224-MCP200. Epub 2008 Aug 26.

Chen Y, Sprung R, Tang Y, et al. Mol Cell Proteomics. 2007, 6(5):812-819. ?


ELISA, WB, IP?

*WB=Western blotting; IP=Immunopreciptation


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