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杭州景杰生物科技有限公司
Anti-propionyl-mono-methyllysine antibody beaded agarose

Cat#:

PTM-683

Ab Type:

P/M

Applications:

IAC IP

Species:

All

Price: (0.5 ml)

34632.00

Price: (0.1 ml)

?10000.00


Product Description

The rabbit-derived polyclonal anti-propionyl-mono-methyllyine antibodies are crosslinked to agarose with stable amide linkages. The antibody agarose conjugated beads is capable of capturing the anti-propionyl-mono-methyllyine peptides. These antibody beads were used in a novel chemical proteomics approach for global screening for mono-methyllysine substrates (1).??


Source/Purification

/

Specificity

/

Recommended Applications

IAP,?

*Immuno affinity precipitation


Recommended antibody dilution

WB: 1:2000

Important

/

Scientific Description

The reversible lysine methylation of proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and gene transcription etc. The lysine residue can be modified at its ?-amino side-chain by mono, di-, and tri-methyl groups. The protein substrates of Kme remain largely unknown due to the relatively low energy radiation emitted by 3H or 14C makes it difficult to detect Kme substrates by a radio labeling approach. To overcome this technical challenge, we develop a chemical proteomics approach for global screening for kme substrates. We first chemically derivatized the mono-methyl ε-amine group of the lysine residue, adding a propionyl moiety. Then, the propionyl mono-methylated peptides were enriched using the pan anti-propionyl mono-methyllysine antibody conjugated agarose beads, followed by HPLC/MS/MS analysis. We have identified 446 Kme sites in 398 proteins, including 3 novel histone Kme marks.??


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



1. Wu Z, Cheng Z, Sun M, Wan X, Liu P, He T, Tan M, Zhao Y. (2014) A chemical proteomics approach for global analysis of lysine mono-methylation. Mol Cell Proteomics.

IAP,?

*Immuno affinity precipitation


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